NSJ Bioreagents

PP2Ac Antibody

Product Code:
 
NSJ-R30249
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
The lyophilized PP2Ac antibody can be stored at 40 to -200. After reconstitution, aliquot and store at -200. Avoid repeated freeze/thaws.
1 / 3
IHC staining of FFPE human breast cancer with PP2Ac antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE rat kidney with PP2Ac antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
3 / 3
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human U-251, 4) rat brain, 5) rat PC-12, 6) mouse lung, 7) mouse liver, 8) mouse brain and 9) mouse NIH 3T3 cell lysate with PP2Ac antibody. Predicted molecular weight ~35 kDa.
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IHC staining of FFPE human breast cancer with PP2Ac antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney with PP2Ac antibody. HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human Caco-2, 3) human U-251, 4) rat brain, 5) rat PC-12, 6) mouse lung, 7) mouse liver, 8) mouse brain and 9) mouse NIH 3T3 cell lysate with PP2Ac antibody. Predicted molecular weight ~35 kDa.

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CodeSizePrice
NSJ-R30249-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Variations in secondary/substrate sensitivities and test protocols may require the PP2Ac antibody to be titrated for optimal performance.
Description:
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of PPP2CA is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human PPP2CA (FTKELDQWIEQLNEC) was used as the immunogen for this PP2Ac antibody (100% homologous in human, mouse and rat).
Limitation:
This PP2Ac antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P67775