PPAR gamma Antibody

Western blot: 0.5-1ug/ml

The stated application concentrations are suggested starting points. Titration of the PPAR gamma antibody may be required due to differences in protocols and secondary/substrate sensitivity.

The peroxisome proliferator-activated receptors are a group of three nuclear receptor isoforms, PPAR gamma, alpha, delta, encoded by different genes. PPARs are ligand-regulated transcription factors that control gene expression by binding to specific response elements (PPREs) within promoters. PPAR gamma is a transcription factor that has a pivotal role in adipocyte differentiation and expression of adipocyte-specific genes. Isoforms 1 and 2 result from alternative splicing and have ligand-dependent and -independent activation domains. PPAR gamma is a member of a family of nuclear receptors/ligand-dependent transcription factors, which bind to hormone response elements on target gene promoters. Ameshima et al.(2003) found that it is abundantly expressed in normal lung tissues, especially in endothelial cells, but that its expression is reduced or absent in the angiogenic plexiform lesions of pulmonary hypertensive lungs and in the vascular lesions of a rat model of severe pulmonary hypertension. And they conclude that fluid shear stress decreases its expression in endothelial cells and that loss of expression characterizes an abnormal, proliferating, apoptosis-resistant endothelial cell phenotype.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Amino acis 45-62 (PHYEDIPFTRTDPVVADY-human) were used as the immunogen for this PPAR gamma antibody.

This PPAR gamma antibody is available for research use only.

Antigen affinity

Human

P37231