NSJ Bioreagents

Ptpn22 Antibody / Protein tyrosine phosphatase non receptor type 22

Product Code:
 
NSJ-RQ4039
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Ptpn22 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
IHC testing of FFPE mouse spleen tissue with Ptpn22 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 4
IHC testing of FFPE rat lymph tissue with Ptpn22 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 4
Western blot testing of rat thymus lysate with Ptpn22 antibody at 0.5ug/ml. Expected molecular weight ~92 kDa, but can be observed at ~105 kDa.
4 / 4
Western blot testing of human 1) SW579, 2) HepG2, 3) placenta, 4) HeLa and 5) SW620 lysate with Ptpn22 antibody at 0.5ug/ml. Expected molecular weight ~92 kDa, but can be observed at ~105 kDa.
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IHC testing of FFPE mouse spleen tissue with Ptpn22 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat lymph tissue with Ptpn22 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of rat thymus lysate with Ptpn22 antibody at 0.5ug/ml. Expected molecular weight ~92 kDa, but can be observed at ~105 kDa.
Western blot testing of human 1) SW579, 2) HepG2, 3) placenta, 4) HeLa and 5) SW620 lysate with Ptpn22 antibody at 0.5ug/ml. Expected molecular weight ~92 kDa, but can be observed at ~105 kDa.

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NSJ-RQ4039-100ug100 ug£535.00
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Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Ptpn22 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Protein tyrosine phosphatase, non-receptor type 22 (lymphoid), also known as PTPN22, is a protein that in humans is encoded by the PTPN22 gene. This gene encodes of member of the non-receptor class 4 subfamily of the protein-tyrosine phosphatase family. The encoded protein is a lymphoid-specific intracellular phosphatase that associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T-cell receptor signaling pathway. Mutations in this gene may be associated with a range of autoimmune disorders including Type 1 Diabetes, rheumatoid arthritis, systemic lupus erythematosus and Graves' disease. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse partial protein corresponding to amino acids M1-Q309 was used as the immunogen for the Ptpn22 antibody.
Limitation:
This Ptpn22 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P29352