NSJ Bioreagents

RFC1 Antibody

Product Code:
 
NSJ-R30806
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution the RFC1 antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with RFC1 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 4
IHC testing of FFPE human breast cancer tissue with RFC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 4
Western blot testing of human 1) 293T, 2) HeLa, 3) HepG2, 4) Raji, 5) K562 and 6) Caco-2 cell lysate with RFC1 antibody. Predicted molecular weight ~128 kDa.
4 / 4
Flow cytometry testing of fixed and permeabilized human HeLa cells with RFC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RFC1 antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with RFC1 antibody (green) and Beta Tubulin mAb (red). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human breast cancer tissue with RFC1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of human 1) 293T, 2) HeLa, 3) HepG2, 4) Raji, 5) K562 and 6) Caco-2 cell lysate with RFC1 antibody. Predicted molecular weight ~128 kDa.
Flow cytometry testing of fixed and permeabilized human HeLa cells with RFC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RFC1 antibody.

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CodeSizePrice
NSJ-R30806-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 5ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the RFC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Replication Factor C, also called Activator-1, is a multimeric primer-recognition protein consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. The human protein was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human RFC1 (NSSRKEDDFKQKQPSKKKR) was used as the immunogen for this RFC1 antibody.
Limitation:
This RFC1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P35251