NSJ Bioreagents

SSH3BP1 Antibody

Product Code:
 
NSJ-R30167
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the SSH3BP1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 4
Western blot testing of 1) rat brain, 2) mouse brain and 3) human ThP-1 lysate with SSH3BP1 antibody. Predicted molecular weight ~55 kDa.
2 / 4
IF: SSH3BP1 antibody testing of rat brain tissue
3 / 4
IHC staining of FFPE rat brain with SSH3BP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE human lung cancer with SSH3BP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of 1) rat brain, 2) mouse brain and 3) human ThP-1 lysate with SSH3BP1 antibody. Predicted molecular weight ~55 kDa.
IF: SSH3BP1 antibody testing of rat brain tissue
IHC staining of FFPE rat brain with SSH3BP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer with SSH3BP1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-R30167-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SSH3BP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
ABI1 is a human homolog of mouse Abl-interactor-1(Abi1), mapped on 10p11.2. ABI1 participates in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor(GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human SSH3BP1 (WYEGVCNRVTGL) was used as the immunogen for this SSH3BP1 antibody.
Limitation:
This SSH3BP1 antibody is available for research use only.
PM_Meta_Key:
Product_Images
PM_Meta_Value:
https://www.nsjbio.com/get_image.php?catalog_no=R30167&type=image1~Immunofluorescent staining of FFPE human intestinal cancer tissue with SSH3BP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.|https://www.nsjbio.com/get_image.php?catalog_no=R30167&type=image2~Immunofluorescent staining of FFPE human breast cancer tissue with SSH3BP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.|https://www.nsjbio.com/get_image.php?catalog_no=R30167&type=image3~Immunofluorescent staining of FFPE human U-2 OS cells with SSH3BP1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.|https://www.nsjbio.com/get_image.php?catalog_no=R30167&type=image4~IHC staining of FFPE human colorectal adenocarcinoma tissue with SSH3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|https://www.nsjbio.com/get_image.php?catalog_no=R30167&type=image5~IHC staining of FFPE human lung cancer tissue with SSH3BP1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.|https://www.nsjbio.com/get_image.php?catalog_no=R30167&type=image6~Western blot testing of 1) human 293T, 2) human MCF7, 3) human U-87 MG, 4) rat brain, 5) rat C-6, 6) mouse brain, 7) mouse Neuro-2a and 8) mouse C2C12 cell lysate with SSH3BP1 antibody. Predicted molecular weight ~55 kDa, commonly observed at 55-65 kDa.|https://www.nsjbio.com/get_image.php?catalog_no=R30167&type=image7~Flow cytometry testing of fixed and permeabilized human MCF7 cells with SSH3BP1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SSH3BP1 antibody.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q8IZP0