NSJ Bioreagents

TCP1 beta Antibody / CCT2

Product Code:
 
NSJ-R32477
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Prior to reconstitution store at 40. After reconstitution the TCP1 beta antibody can be stored for up to one month at 40. For long-term aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Western blot testing of 1) rat brain, 2) rat spleen, 3) rat C6, 4) rat PC-12, 5) mouse brain, 6) mouse spleen, 7) mouse NIH 3T3 and 8) mouse RAW264.7 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.
2 / 7
Western blot testing of human 1) A549, 2) RT4, 3) Caco-2, 4) 293T, 5) MCF7, 6) HEL, 7) Raji and 8) SW620 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.
3 / 7
IHC testing of FFPE human testis tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
4 / 7
IHC testing of FFPE mouse gastric tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
5 / 7
IHC testing of FFPE rat gastric tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
6 / 7
Immunofluorescent staining of FFPE human U-2 OS cells with TCP1 beta antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of human U-251 cells with TCP1 beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 beta antibody.
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Western blot testing of 1) rat brain, 2) rat spleen, 3) rat C6, 4) rat PC-12, 5) mouse brain, 6) mouse spleen, 7) mouse NIH 3T3 and 8) mouse RAW264.7 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.
Western blot testing of human 1) A549, 2) RT4, 3) Caco-2, 4) 293T, 5) MCF7, 6) HEL, 7) Raji and 8) SW620 cell lysate with TCP1 beta antibody. Predicted molecular weight ~57 kDa.
IHC testing of FFPE human testis tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE mouse gastric tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE rat gastric tissue with TCP1 beta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
Immunofluorescent staining of FFPE human U-2 OS cells with TCP1 beta antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-251 cells with TCP1 beta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 beta antibody.

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CodeSizePrice
NSJ-R32477-100ug100 ug£535.00
Quantity:
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
T-complex protein 1 subunit beta (TCP1 beta) is a protein that in humans is encoded by the CCT2 gene. The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids E414-C535 from the human protein were used as the immunogen for the TCP1 beta antibody.
Limitation:
This TCP1 beta antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P78371