NSJ Bioreagents

TRAF2 Antibody

Product Code:
 
NSJ-R32205
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the TRAF2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 2
Western blot testing of human 1) HeLa and 2) Jurkat lysate with TRAF2 antibody. Predicted/expected molecular weight ~56 kDa.~
2 / 2
IHC testing of FFPE human tonsil with TRAF2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 2
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Western blot testing of human 1) HeLa and 2) Jurkat lysate with TRAF2 antibody. Predicted/expected molecular weight ~56 kDa.~
IHC testing of FFPE human tonsil with TRAF2 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 2

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NSJ-R32205-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the TRAF2 antibody should be determined by the researcher.
Description:
TRAF2 (TNF Receptor-Associated Factor 2), also called TRAP, is a protein that in humans is encoded by the TRAF2 gene. The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. It is a common signal transducer for TNFR2 and CD40 that mediates activation of NF-kappa-B. Rothe et al. (1996) identified ITRAF, which binds to TRAF1, TRAF2, and TRAF3, and that when overexpressed inhibits TRAF2-mediated NF-kappa-B activation. They proposed that ITRAF is an inhibitor of TRAF function that regulates TRAF protein activity by sequestering TRAFs in a latent state in the cytoplasm. Kanamori et al. (2002) found that mouse Traf2 interacted directly with T2bp, and they presented evidence that T2BP is involved in TNF-mediated signaling by its interaction with TRAF2.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RLDQDKIEALSSKVQQLERSIGLKDLAMADLE of human TRAF2 were used as the immunogen for the TRAF2 antibody.
Limitation:
This TRAF2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q12933