NSJ Bioreagents

Transferrin Receptor Antibody

Product Code:
 
NSJ-R31646
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Transferrin Receptor antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 7
Immunofluorescent staining of FFPE human A431 cells with Transferrin Receptor antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 7
Western blot testing of 1) human HeLa, 2) human K562, 3) human HT1080, 4) rat thymus, 5) mouse RAW264.7 and 6) mouse SP2/0 cell lysate with Transferrin Receptor antibody. Predicted molecular weight 85~95 kDa.
3 / 7
IHC-P: Transferrin Receptor antibody testing of human placenta tissue
4 / 7
IHC staining of frozen human placenta with Transferrin Receptor antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 7
Flow cytometry testing of human SiHa cells with Transferrin Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Transferrin Receptor antibody.
6 / 7
Flow cytometry testing of human U-87 MG cells with Transferrin Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Transferrin Receptor antibody.
7 / 7
Immunofluorescent staining of FFPE human placental tissue with Transferrin Receptor antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human A431 cells with Transferrin Receptor antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) human HeLa, 2) human K562, 3) human HT1080, 4) rat thymus, 5) mouse RAW264.7 and 6) mouse SP2/0 cell lysate with Transferrin Receptor antibody. Predicted molecular weight 85~95 kDa.
IHC-P: Transferrin Receptor antibody testing of human placenta tissue
IHC staining of frozen human placenta with Transferrin Receptor antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human SiHa cells with Transferrin Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Transferrin Receptor antibody.
Flow cytometry testing of human U-87 MG cells with Transferrin Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Transferrin Receptor antibody.
Immunofluorescent staining of FFPE human placental tissue with Transferrin Receptor antibody (red) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH8 EDTA buffer for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-R31646-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Transferrin Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. It is mapped to 3q29. TFRC is a transmembrane glycoprotein composed of two disulfide-linked monomers joined by two disulfide bonds. Expression of human TfR1 in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses. TfR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. It is required for iron delivery from transferrin to cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
7037
Immunogen:
Human partial recombinant protein (AA 1-198) was used as the immunogen for this Transferrin Receptor antibody.
Limitation:
This Transferrin Receptor antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human